Reagents recommended by Ellyn Daugherty

Reagents recommended for Biotechnology, Science for the New Millennium 
Catalog Number Description Size  Cost  Used in Chapter
786-007 Bovine Y-Globulin Protein Standard [2mg/ml] 2 x 5ml  $       49.00 Multiple 
786-016 SDS [20%] 500ml  $       67.00 Multiple 
786-025 SDS-PAGE Sample Loading Buffer [2X] 2.5ml  $       19.00 Multiple 
786-029 SDS-PAGE Running Buffer [10X] 1 Liter  $       37.00 Multiple 
786-029G SDS-PAGE Running Buffer [10X] 1 Gallon  $     107.00 Multiple 
786-292 Water, Molecular Grade 500ml  $       22.00 Multiple 
786-35 LabSafe GEL Blue™ 1 Liter  $       98.00 Multiple 
786-35G LabSafe GEL Blue™ 1 Gallon  $     236.00 Multiple 
786-495 Coomassie Brilliant Blue R-250 Dye 10g  $       39.00 Multiple 
786-701 SDS-PAGE Sample Loading Buffer [6X] 25ml  $       33.00 Multiple 
786-856 DNAmark  100bp Plus Ladder 50ug  $       51.00 Multiple 
BTNM-0007 Phosphate Buffered Saline (PBS) 10X 1 L  $       51.00 Multiple 
BTNM-0008 Agarose RA 25g  $       51.00 Multiple 
BTNM-0009 Agarose RA 100g  $     140.00 Multiple 
BTNM-0010 alpha-Amylase 25g  $       22.00 Multiple 
BTNM-0012 Bacto agar 100g  $       88.00 Multiple 
BTNM-0013 BamHI Enzyme, 2500 U, with 10X reaction buffer 2500U  $       47.00 Multiple 
BTNM-0014 BCIP(X-Phosphate)/NBT COLOR 1.25/2.5 mL 15ml  $       48.00 Multiple 
BTNM-0015 Benedict's Qualitative Solution 500 mL  $       11.00 Multiple 
BTNM-0018 Bradford Reagent [5X] 500ml  $     128.00 Multiple 
BTNM-0019 Bromophenol Blue, Crystals 5g  $       14.00 Multiple 
BTNM-0020 BSA, Albumin, Bovine Serum 25g  $       83.00 Multiple 
BTNM-0030 DTT [0.1M] 7.7mg  $       26.00 Multiple 
BTNM-0033 EDTA, Disodium salt, Lab Grade 100g  $       21.00 Multiple 
BTNM-0034 Ethidium Bromide, 5 mg/mL 1mL  $       18.00 Multiple 
BTNM-0035 Filter Paper, Whatman Grade #1, MediumFlow Rate, 125 mm 100/pk  $       24.00 Multiple 
BTNM-0036 Filtering Flasks, 250 mL, 0.2 µm, CN 12/cs  $     152.00 Multiple 
BTNM-0042 Lambda DNA, cut w/HinDIII, 1x100 µg, 0.1 µg/µL 100µg  $       72.00 Multiple 
BTNM-0043 Lambda DNA, uncut 250µg  $     128.00 Multiple 
BTNM-0044 LB Agar Base, Lennox 500 g  $     117.00 Multiple 
BTNM-0045 LB Broth Base, Lennox 500 g  $       66.00 Multiple 
BTNM-0046 Lid Lock, Mulit color with handle 100/pk  $       48.00 Multiple 
BTNM-0047 Lugol's Iodine Solution, 4% 500 mL  $       18.00 Multiple 
BTNM-0054-NG PAGE gel, NuView, 10% TG, 10 well  10  $     204.00 Multiple 
BTNM-0055 PageMark Protein MW Marker, tri-color, Prestained 250µL, 50 loads  $       71.00 Multiple 
BTNM-0060 pH Buffer, Blue, pH 10.0 500 mL  $       12.00 Multiple 
BTNM-0061 pH Buffer, Red pH 4.0, 500 mL  $       12.00 Multiple 
BTNM-0062 pH Buffer, Yellow, pH 7.0 500 mL  $       12.00 Multiple 
BTNM-0063 Porcine Serum 100 mL  $       63.00 Multiple 
BTNM-0069 Sodium Hydroxide pellets 500g  $       28.00 Multiple 
BTNM-0071 Starch, Soluble, Reagent Grade 500g  $       89.00 Multiple 
BTNM-0073 Syringe Filters  50  $     159.00 Multiple 
BTNM-0084 Glycerol DNA Loading Dye Buffer 10X 5 mL  $       18.00 Multiple 
BTNM-0085 20X LB conductive medium (Better Faster Media) 1L  $       68.00 Multiple 
BTNM-0086 20X LB conductive medium (Better Faster Media) 1 Gallon  $     198.00 Multiple 
R023 TAE [50X] (Tris-Acetate-EDTA) 1 Liter  $       59.00 Multiple 
RC-028 Boric Acid 500g  $       37.00 Multiple 
RC-030 Calcium Chloride,Dihydrate (CaCl2.2H2O) 500g  $       45.00 Multiple 
RC-093 Sodium Chloride 500g  $       37.00 Multiple 
RC-095 Sodium Phosphate (Dibasic) 500g  $       59.00 Multiple 
RC-097 Sodium Phosphate (Monobasic) 500g  $       37.00 Multiple 
RC-099 Sucrose 500g  $       38.00 Multiple 
RC-105 Tris Base 500g  $       54.00 Multiple 
RC-125 Sodium Carbonate; Na2CO3 (anhydrous) 500g  $       48.00 Multiple 
RC-118 Mineral Oil 500ml  $       60.00 Multiple 
RC-123 Potassium Chloride; KCl 500g  $       57.00 Multiple 
BTNM-0025 Chymosin, (Fermentation Produced) 100 mL  $       25.00 1
BTNM-0067 Chymosin, (Rennin), Bovine, Lab Grade 25g  $       43.00 1
BTNM-0095 Yeast Malt Agar 125ml  $         9.00 2
BTNM-0065 Potato Dextrose Agar, 125 mL bottles 6 x 125ml  $       29.00 2
BTNM-0001 12-Well Spot Plate 12/pk  $       22.00 2,11
BTNM-0082 Yeast active dry powder 100g  $       15.00 2,4
BTNM-0093 E. coli, DH5 alpha, agar stab 1ml  $       29.00 2,4,8,12
RC-052 Gelatin (Reagent Grade) 100g  $       36.00 2,6
BTNM-0003 96-well Micro-titer Plate, general use 25/pk  $       59.00 3,11
BTNM-0075 TBE Buffer Concentrate, 1X 1L  $       29.00 3,13
R003 TE Buffer [100X]  100ml  $       29.00 3,4,11
BTNM-0083 Glucose Test Strips 100 Strips  $       47.00 3,6,9,14
BTNM-0066 Proteinase K 25ml, 1mg/ml  $     106.00 4,5,11
RC-107 Tris HCl 500g  $       98.00 4,6,13
BTNM-0092 pAmylase2014 [0.2μg/μl] 0.2ml  $     139.00 4,7,8,13
786-339 XIT™ Genomic DNA from Gram Positive Bacteria For 25ml Culture  $     200.00 4
BTNM-0029 DNA, Salmon Testes 1 g  $     164.00 4
BTNM-0077 Triton X-100, 2% 20 mL  $       46.00 4
BTNM-0068 RNase, 5mg/mL 1ml  $       65.00 4,8,11
BTNM-0058 Pectinase 25g  $       77.00 5
BTNM-0022 Cellulase, Aspergillus niger 25g  $       43.00 5,14
BC98 β-Mercaptoethanol 100ml  $       36.00 5,6,9,11
BTNM-0070 Starch, Modified Soluble, Alfa Aesar® 25g  $       59.00 6
BTNM-0080 Tween-20 10% solution 20 mL  $       46.00 6,10,11
BTNM-0004 96-well Micro-titer Plate, Maxisorp for ELISA 25/pk  $     175.00 6,11
BTNM-0052 Nitrocellulose membrane (7.5cm x 8.5cm) 20 precut sheets  $       70.00 6,11
BTNM-0076 TMB Solution 100 mL  $       58.00 6,11
RC-054 Glycine 500g  $       39.00 6,11
BTNM-0037 Glycerol, sterile 25ml  $       27.00 6,11
BTNM-0002 24-Well Microtiter Plate w/ Lid 50/cs  $     144.00 6,9
BTNM-0096 Casein Powder 100g  $       14.00 7
BTNM-0059 Nucleic dotMETRIC Basic Kit, G-bioscience 1 kit  $     231.00 8,11,13
A051-B Ampicillin  5g  $       24.00 8,9,12
786-362 GET™ Plasmid DNA Miniprep Kit 100 Preps  $     131.00 8
BTNM-0032 EcoRI enzyme, 5000 U, with 10X reaction buffer 5,000U  $       78.00 8
BTNM-0038 HinDIII restriction enzyme, 5000 U, with 10X Reaction Buffer 5,000U  $       78.00 8
BTNM-0091 pAmylase2014 [0.005μg/μl] 0.2ml  $       76.00 8
P313 Potassium Acetate 100g  $       24.00 8
BTNM-0027 DEAE Agarose 50 mL  $     120.00 9
BTNM-0048 Lysozyme, Reagent Grade 1 g  $       47.00 9
BTNM-0049 Murashige and Skoog Multiplication Medium 1L  $         8.00 10
BTNM-0050 Murashige and Skoog pretransplant medium for African Violets 1L  $         8.00 10
BTNM-0051 Murashige and Skoog Basal w/ Gamborg Vitamins 1L  $         8.00 10
P317 Potassium Hydroxide (KOH) 100g  $       38.00 10
786-298 XIT™ Genomic DNA from Plant Tissue 25g Tissue  $     209.00 11
786-724 Spin Column, 3ml 25 columns  $       60.00 11
BTNM-0005 AB: Anti-Pig IgG conjugated with HRP 100µl  $       25.00 11
BTNM-0006 AB:Rabbit Anti-Amylase IgG Antibody-HRP, polyclonal, 10mg/ml 100µg  $     220.00 11
BTNM-0017 Actin from Rabbit Muscle 1mg  $     186.00 11
BTNM-0026 Cow Serum 100 mL  $       47.00 11
BTNM-0041 Kanamycin Sulfate 25 g  $       93.00 11
BTNM-0081 X-Gluc, 5-Bromo-4-Chloro-3-IND 100mg  $     138.00 11
BTNM-0090 GET™ Plant DNA Template 20 Preps  $       81.00 11
R004 Magnesium Chloride [1M] 100ml  $       29.00 11
RC-083 Potassium Phosphate (Monobasic) 500g  $       46.00 11
786-443 Deoxynucleotide Mix [10mM] 5µmole  $       40.00 13
786-449 Taq Polymerase 2X Mastermix 100 Reactions  $       70.00 13
BE-305 Polymerase Chain Reaction For 6 groups of 4-5 or 24-30 students.  $     237.00 13
BTNM-0016 Biotin-tagged Alkaline Phosphatase [0.38mg/ml] 200µl  $       88.00 13
BTNM-0024 Chelex Beads, 0.4 g w/ buffer 10 mL  $       43.00 13
BTNM-0039 Hybond N+ Membrane 20 sheets  $     208.00 13
BTNM-0040 DNA Synthesis Kit For 32 groups  $     128.00 13
BTNM-0072 Streptavidin 1mg/ml 1ml  $       88.00 13
BTNM-0074 Taq DNA polymerase Std reaction buffer 400U  $       47.00 13
BTNM-0078 Tryptic Soy Agar 500g  $       65.00 13
BTNM-0079 Tryptic Soy Broth 500g  $       54.00 13
BTNM-0087 20X LA® conductive medium (Faster Better Media) 1 Gallon  $     141.00 13
RC-068 Magnesium Chloride 100g  $       36.00 13
BKC-17 DMSO, anhydrous 50ml  $       60.00 14
BTNM-0021 Cellulase, Trichoderma reesei 25g  $       43.00 14
BTNM-0023 Cellulose, Laboratory Grade 25g  $       15.00 14
BTNM-0028 1,5-Diphenylcarbazide, 99% 25g  $       69.00 14
BTNM-0064 Potassium chromate 100g  $       50.00 14